Reaction Behavior of [1,3-Diethyl-4,5-diphenyl-1H-imidazol-2-ylidene] Containing Gold(I/III) Complexes against Ingredients of the Cell Culture Medium and the Meaning on the Potential Use for Cancer Eradication Therapy

The reactivities of halido[1,3-diethyl-4,5-diphenyl-1H-imidazol-2-ylidene]gold(I) (chlorido (5), bromido (6), iodido (7)), bis[1,3-diethyl-4,5-diphenyl-1H-imidazol-2-ylidene]gold(I) (8), and bis[1,3-diethyl-4,5-diphenyl-1H-imidazol-2-ylidene]dihalidogold(III) (chlorido (9), bromido (10), iodido (11)) complexes against ingredients of the cell culture medium were analyzed by HPLC. The degradation in the RPMI 1640 medium was studied, too. Complex 6 quantitatively reacted with chloride to 5, while 7 showed additionally ligand scrambling to 8. Interactions with non-thiol containing amino acids could not be detected. However, glutathione (GSH) reacted immediately with 5 and 6 yielding the (NHC)gold(I)-GSH complex 12. The most active complex 8 was stable under in vitro conditions and strongly participated on the biological effects of 7. The gold(III) species 9–11 were completely reduced by GSH to 8 and are prodrugs. All complexes were tested for inhibitory effects in Cisplatin-resistant cells, as well as against cancer stem cell-enriched cell lines and showed excellent activity. Such compounds are of utmost interest for the therapy of drug-resistant tumors.


■ INTRODUCTION
The need for new and improved drugs in tumor therapy is growing rapidly, because of the occurrence of an increased number of tumoral diseases that are resistant to conventional anticancer drugs. Especially, metallodrugs are promising candidates to circumvent acquired and intrinsic resistance. 1−6 In particular, gold complexes were established as effective cytostatics, since the approval of Auranofin in 1985 as a drug against rheumatoid arthritis. 7−11 Auranofin is now approved by the FDA for phase-II clinical trials in cancer therapy. 12 During the past years, gold containing drug candidates with N-heterocyclic carbenes (NHC) as ligands received high attention, because of their easy synthesis and good biological activity. 8,13−16 Antiproliferative effects of (NHC)gold(I)-X complexes where X represents a leaving group are well documented. This particularly applies to halido derivatives. While the meaning of halido leaving groups at platinum(II) complexes on the reactivity in water and thus also on the cytotoxicity has been intensively studied, 17,18 comparable investigations with (NHC)gold(I)-X (X = Cl, Br, I) complexes are rather rare. In aqueous solution, nucleophiles can force ligand exchange reactions, but ligand scrambling is observed, too. 19 Chloride, present in cell culture media, transforms the bromido complex to the chlorido species as demonstrated in time-dependent experiments on the example of halido[3-ethyl-4-(4-methoxyphenyl)-5-(2-methoxypyridin-5-yl)-1-propyl-1Himidazol-2-ylidene]gold(I) complexes (mono-NHC-Au(I)-X, Chart 1). Kinetic reactions with other nucleophiles of the media, e.g., amino acids or glutathione (GSH), were not investigated yet, although such reaction is described for other metal complexes, including Cisplatin. 20−22 However, even for Cisplatin, this issue has not yet been fully elucidated as recently discussed by Hall. 23 Unlike platinum(II) complexes, aquation is not the preferred reaction of mono-NHC-Au(I)-X complexes in water. Instead, they show partially ligand scrambling resulting in the cationic [(NHC) 2 Au(I)] + complex (bis-NHC-Au(I), Chart 1) and [Au(I)X 2 ] − ion. The latter finally decomposes to Au(0). 24,25 Especially, the mono-NHC-Au(I)-I complex displayed this reaction during the first minutes of incubation.
Such transformations are of high importance for the interpretation of the biological results, because there are several clues that the antiproliferative activity of [(NHC) 2 Au-(I)] + species is generally more than 5-fold higher than that of the related (NHC)gold(I)-X (X = Cl, Br, I) complexes. 26 −31 In water-free organic solvent, e.g., dimethylformamide (DMF), the mono-NHC-Au(I)-X complexes (Chart 1) were stable for 72 h (degradation <2%), 24 so stock solutions for the in vitro testing can be prepared. The final concentrations for the cell culture experiments were achieved by dilution with the respective medium, to realize a maximum amount of organic solvent of 0.1%.
It is assumed that the first contact with the medium started the ligand scrambling of mono-NHC-Au(I)-X and present nucleophiles force the degradation further. Therefore, we investigated in a preliminary study the stability of the complexes in acetonitrile/water = 50/50 (v/v) in the presence of 0.9% NaCl. To simulate the reaction with medium ingredients, the complexes were treated with a 20-fold excess of iodide as a model nucleophile.
Mono-NHC-Au(I)-Br and mono-NHC-Au(I)-I rapidly reacted in NaCl solution to mono-NHC-Au(I)-Cl, but also underwent a distinct degradation to bis-NHC-Au(I) (Chart 1). 19,27,32 Iodide as the nucleophile led to a fast reaction to mono-NHC-Au(I)-I and subsequently to an increased formation of bis-NHC-Au(I).
It is very likely that reaction products reduce on the one hand the concentration of the investigated complex and on the other hand have antiproliferative activity themselves.
The RPMI 1640 cell culture medium used in this study contains, besides numerous L-amino acids, vitamins such as biotin, i-inositol, or vitamin B12. Also present is a large amount of glucose and chloride containing inorganic salts (sodium and potassium chloride) as well as the tripeptide GSH.
In this study, we reacted the halido(NHC)gold(I) complexes 5−7 with main ingredients of the medium, e.g., chloride and amino acids. The products, in particular, the [(NHC) 2 Au(I)] + species, were time-dependent quantified by high-pressure liquid chromatography (HPLC) analysis.
Besides the reaction with each nucleophile, the degradation of the complexes in a complete RPMI 1640 cell culture medium was monitored.
Another relevant aspect is the presence of GSH in the cell culture medium. The glutathione ″supersystem″ causes detoxification due to binding of GSH to heavy metals and other toxins. 33−35 Since it is well known that gold complexes have a high affinity for thiol-bearing compounds, 36 we also quantified the reaction of the (NHC)gold complexes 5−7 with GSH.
Of further interest is the redox behavior of GSH, which allows a reduction of Au(III) to Au(I). Therefore, we included in this study [(NHC) 2 Au(III)X 2 ] + complexes with X = Cl (9), Br (10), and I (11) and determined their stability in the presence of GSH.
Furthermore, the complexes 5−11 were investigated for antiproliferative effects in various cell lines, sensitive and resistant to known cytostatics, as well as in cancer cell lines with enriched proportions of cells with stem cell characteristics (cancer stem cells, CSC).
The influence of the halido ligands on the NHC resonances in the 1 H NMR spectra of 5−11 (Figures S8−S14) was only marginal and did not allow a structural discrimination. However, this is possible based on the resonances of the C2 carbon directly bound to gold(I/III). From the 13 C NMR spectra ( Figures S15−S21), it is obvious that the chemical shift depends on the bound halide and oxidation state of the metal center.
X-Ray crystallography confirmed the structure of the complexes 5−11 ( Figure 1 and Figures S22−S25). Crystals were grown by slow crystallization over several days from an ACN solution (for crystallographic data, see Tables S1−S8).
The spatial structures of the NHC ligand and the length of the NHC-gold bond (1.995−2.028 Å) were nearly identical in all (NHC)Au(I)-X complexes, while the Au(I)-X distance (2.299 Å (5), 2.394 Å (6), and 2.584 Å (7)) increased with the atomic radius of the halide. The complexes formed slightly distorted columnar structures with Au(I)-Au(I) distances of 3.532 and 3.579 Å in 5 and 6, as well as of 4.011 Å in 7. These values point to weak aurophilic interactions.
The ligands took an orientation, in which the NHC moieties are perpendicularly arranged.
Oxidation to Au(III) and coordination of two halido ligands resulted in a square planar environment at the metal center. The NHCs were aligned perpendicular to this plane due to the steric repulsion with the halido ligands.
Reactivity Studies. In a previous study, we established an HPLC system, which allows the quantification of mono-NHC-Au(I)-X (X = Cl, Br, and I) in the presence of nucleophiles. This method was adapted to the analysis of the complexes 5− 11.
As mentioned above, the RPMI 1640 medium contains as nucleophiles besides chloride a variety of amino acids and Journal of Medicinal Chemistry pubs.acs.org/jmc Article GSH, which can coordinate to the metal or be involved in the redox reaction with gold(I/III). All nucleophiles (chloride, amino acids, and GSH) were separately reacted with 5−11 in ACN/water = 50/50 (v/v) mixtures at a complex concentration 0.5 mM. This solvent composition was chosen to prevent precipitation of the complex and the formed degradation products.
As a source for chloride, a NaCl (12.0 g/L)/KCl (0.8 g/L) solution was prepared. After mixing with ACN (50/50 (v/v)), the concentration corresponds to that in the medium.
Amino acids, as well as GSH, were used in 20-fold excess, as realized at a complex concentration of 10 μM in the cell culture medium. Thereto, the respective complex, dissolved in ACN (1 mM), was combined with the same amount of aqueous solution of the nucleophile (20 mM).
The mixture was then incubated for 24 h at rt and monitored at various time points via HPLC using an RP-C18 column and gradient elution (70/30 (v/v) to 90/10 (v/v)) of ACN/water (0.1% trifluoroacetic acid (TFA)).
It must be mentioned that ACN can act as a weak nucleophile and is able to displace the halide from the gold(I) center of (NHC)Au(I)-X complexes, resulting in the ((NHC)-Au(I)-ACN) species. This reaction product would be the same for 5−7.
However, the chromatograms of the complexes in ACN solution (Figures S1−S3) show peaks with different retention times (5: t R = 5.13 min, 6: t R = 5.92 min, and 7: t R = 7.00 min). An additional peak probably caused by (NHC)Au(I)-ACN is not present. Transformation to the Au(I)-ACN can therefore be excluded.
A further clue that the peak at t R = 5.13 min in the chromatograms of 5 results from (NHC)Au(I)-Cl and not from (NHC)Au(I)-ACN can be driven from Figure 2. High chloride concentrations prevent the substitution of the chloride leaving group by weak nucleophiles. As depicted in Figure 2, only a small amount (0.9%) of 5 underwent ligand scrambling to the [(NHC) 2 Au(I)] + complex 8 during 24 h of incubation. No further peak of a possible (NHC)Au(I)-ACN species was observed.
In contrast, the bromido derivative 6 reacted quantitatively to 5 immediately after mixture of complex and chloride solution (Figure 2A), followed by the same degradation profile as observed for 5 ( Figure 2B).
Incubation of 8 under the same conditions did not result in any degradation. This finding proves that it is the most stable complex and that its formation from (NHC)Au(I)-X complexes 5−7 is irreversible.
In the next step, the stability of the gold(III) species 9−11 in the presence of chloride was investigated ( Figure 2).
Ligand exchange at the dichlorido complex 9 was not observed. However, it was slightly reduced to 8 during 24 h of incubation (by 12.5%).
The iodido ligands at the gold(III) center of 11 stabilized the complex. In chloride containing solution, only traces of 9 (0.4%) were detected at t 0h without further degradation.
Next, it was evaluated on the example of 7, whether (NHC)Au(I)-X complexes generally interact with amino acids available in the medium.
The HPLC analysis indicated that 7 did not react with the amino acids. Only ligand scrambling to 8 occurred. Timedependent investigations pointed out that this reaction mostly finished within the first 30 min of incubation ( Figure 3). Only  in pure ACN/water and in the presence of glutamic acid (Glu) or methionine (Met), a further degradation to 8 took place (t 24h : Glu (31.6%), Met (27.5%), and water (31.2%)).
Interestingly, arginine (Arg) diminished the formation of 8. After 30 min, the ratio of 7/8 was 90/10 and remained stable until 24 h of incubation ( Figure 3 and Figure S26).
The most important bionucleophile in the medium represents GSH, which is a strong nucleophile and strong reductant due to its cysteine moiety. It was incubated together with the respective complex (5−11), and the results are depicted in Figure 4.
The used HPLC method allowed the discrimination of GSH from its oxidized form GSSG as well as 5−11 from the (NHC)Au(I)-GSH adduct 12.
The chlorido complex 5, as well as the bromido complex 6, reacted quantitatively with GSH in a substitution reaction. Already after 1.5 min (t 0h , Figure 4A), only 12 was observed, without further degradation (t 24h , Figure 4B). Redox reactions did not occur.
In contrast, GSH displaced iodide from 7 (→12) only in a proportion of 9.7% (t 0h , Figure 4A), which decreased during 24 h to 4.7%, while 9.8% of 8 was formed ( Figure 4B). Time-dependent analysis indicated that ligand scrambling of 7 started after 1 h. At the same time, the amount of 12 diminished ( Figure S27).
GSH caused only substitution reactions, without reduction of Au(I) to Au(0). Other redox agents, too, did not affect the (NHC)Au(I)-X complexes. For instance, complex 5 incubated with sodium ascorbate or NADPH for 24 h showed only marginal ligand scrambling to 8 (ascorbate: 3.4%; NADPH: 5.6%, Figures S28 and S29). Neither reduction to gold(0), release of the NHC ligand, nor coordination to the gold(I) center occurred.
In the next step, the influence of reductive agents on the gold(III) complexes 9−11 was studied on the example of GSH.
Already the first contact (t 0h ) with GSH led to a quantitative reduction to 8 and formation of GSSG ( Figure 4).
These results clearly demonstrates that (1) the reduction of [(NHC) 2 Au(III)X 2 ] + complexes is independent on the coordinated halides, and (2) the fast reduction to [(NHC) 2 Au-(I)] + implicates that 8 might be the biologically active form of 9−11, why examinations in the complete medium are necessary (see below).
The reactions of (NHC)gold(I)-X and [(NHC) 2 Au(III)-X 2 ] + complexes with GSH were also investigated on the example of 5 and 11 by HR-MS.
In addition to the expected reaction product 12 at m/z 780 (for confirmation via HCD fragmentation see Figure S30), also, higher adducts were detected after 24 h ( Figure 5). The doubly charged ion with m/z 626 ((NHC) 2 Au 2 GSH) and the positive ion with m/z 1252 ((NHC) 2 Au 2 GS − ) correspond to a T-shaped intermediate similar to that identified in the watermediated scrambling reaction of mono-NHC-Au(I)-X complexes. 25 HCD fragmentation of the ion m/z 626 ( Figure S31) led among others to a species with m/z 749, which agrees with the [(NHC) 2 Au(I)] + complex 8, confirming the assumed structure with aurophilic interactions. Table S9 lists all identified ions. These findings document that 12 is capable for ligand scrambling.
To study the degradation in the complete cell culture medium, RPMI 1640 (without fetal calf serum (FCS)) was combined with the respective ACN solution of 5−11 in a 50/ 50 (v/v) ratio. Sample preparation and HPLC analysis were performed as described above. The results are depicted in Figure 6.
The reduced transformation (compared to the direct reaction with GSH) might be the consequence of the high chloride concentration in the medium. To confirm this assumption, 5 was incubated in a control experiment with 20 equiv of GSH (as described above) in phosphate buffered saline (PBS). At t 0h , 65.7% of 12 was formed. The amount increased during 24 h to 68.0% of 12 and 2.1% of 8 appeared ( Figure S33). These results points to an inhibitory effect of chloride on the substitution reaction of (NHC)gold(I)-Cl complexes.
The iodido complex 7 was more stable in the RPMI 1640 medium and transformed at t 0h only by 14.3% to 5 and by 0.8% to 8. During 24 h, the amount of 5 remained constant, but 8 increased to 23.1%. No GSH-adduct 12 could be detected.
As expected from the reactivity studies, complex 8 was stable in RPMI 1640 during 24 h of incubation.
The gold(III) complexes 9−11 strongly degraded in the medium. Complex 9 was reduced immediately after contact with the medium to 8 by 5.0% and during 24 h by 36.3%.
In case of 10, additionally, a fast Br/Cl exchange took place. At t 0h , 42.3% of 9 and 9.3% of 8 were formed. During 24 h, the amounts of gold(III) complexes 9 and 10 decreased to 18.2 and 10.7%, respectively, in favor to the gold(I) species 8 (71.1%). In contrast, the solution of 11 contained at t 0h only the [(NHC) 2 Au(III)I 2 ] + complex, which then completely converted to 8 in the redox reaction with GSH within 8 h ( Figure 6B and Figure S34).
In a final experiment, the influence of proteins on the degradation and free available (NHC)Au(I)-X complexes was studied on the example of the iodido complex 7.
The complex (conc. 30 μM) dissolved in RPMI 1640 medium supplemented with 10% FCS was incubated for 4 h at 37°C in the dark. Subsequently, the proteins were precipitated with ACN and the supernatant was dried by lyophilization. The remaining complexes were extracted from the lyophilizate with DCM. The organic layer was evaporated, and the residue was finally dissolved in 1 mL of ACN. This solution was then analyzed by HPLC as described above.
It is obvious from Figure 7 that, besides 7, the chlorido complex 5 and the cationic complex 8 were present as a free fraction. From the peak areas, the concentrations were calculated with 10.12 μM (5), 0.78 μM (7), and 2.14 μM (8). This means that about 50% of the complexes were protein-bound and were separated by protein precipitation with ACN.
In conclusion, the reactivity studies clearly demonstrate that (NHC)Au(I)-X complexes converted in the cell culture medium dependent on the bound halide.
The chlorido complex 5 was very stable in aqueous solution, while the bromido derivative 6 was rapidly and nearly quantitatively transformed to 5. In both cases, marginal ligand scrambling to the [(NHC) 2 Au(I)] + species 8 took place, whose participation on the biological activity cannot be excluded.
A more complicated degradation profile showed the iodido complex 7. Besides the initial complex 7, 5 and 8 were built at  The gold(III) complexes 9−11 were not stable in the medium. They undergo halide exchange reactions and reduction to 8.
The [(NHC) 2 Au(I)] + species 8 was the most stable complex among the tested compounds without degradation in the cell culture medium.
Biological Activity. Effects against Wild-Type and Resistant Cancer Cells. In vitro cytotoxicity assays were performed to get an insight into the antitumor activity of the (NHC)gold complexes. Complexes 5−11, the established antitumor drug Cisplatin as well as Auranofin, used as a reference for a gold containing drug, were tested in different wild-type and corresponding resistant cancer cell lines. The influence on the metabolic activity was quantified in a modified MTT assay. It involves the conversion of the water-soluble yellow dye MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to an insoluble purple formazan by the action of mitochondrial reductase. Formazan is then solubilized in dimethyl sulfoxide (DMSO), and the concentration is determined by optical density at 570 nm.
The complexes 5 and 6 were low active in the lung cancer cell line A549, the Taxol-resistant subclone A549-R, and chronic myelogenous leukemia(CML) cell line K562 and its Doxorubicin-resistant subclone K562-R ( Table 1). The IC 50 values of both complexes were higher than 10 μM. However, the complexes reduced the viability of human MCF-7 breast cancer cells and the Tamoxifen-resistant subline (MCF-7TamR) with IC 50 = 4.54−5.76 μM ( Table 1). The comparable results of both complexes might be the consequence of the degradation of 6 to 5 in the cell culture medium.
The higher activity in hormone-dependent breast cancer cells points to the carrier function of the 1,3-diethyl-4,5diphenyl-1H-imidazol-2-ylidene moiety as related imidazoles were identified as drugs interfering with the estrogen receptor pathway. 43,44 This finding will be evaluated more detailed in a forthcoming structure−activity relationship study.
The [(NHC) 2 Au(I)] + complex 8 was the derivative, which reduced the metabolic activity most effectively in the low nanomolar range, independent on the cell line used (IC 50 = 0.14−1.79 μM, Table 1). The complex was 10-to 20-fold higher active than Cisplatin or Auranofin.
Complex 8 is the one with the highest stability, without transformation under cell culture conditions, why the effects can unequivocally be assigned to this species.
The gold(III) derivatives 9−11 (IC 50 = 0.18−2.26 μM, Table 1) were comparably active as 8. Since it was demonstrated that GSH present in the medium reduces gold(III) to gold(I), it can be assumed that 9−11 are prodrugs and 8 was the active ingredient.
8 was also involved in the activity of 7. The latter is partially transformed in a ligand scrambling reaction to 8 and in a substitution reaction to 5 as discussed above. Therefore, it is very likely that the effects observed for 7 caused at least three compounds.
The complexes 5−11 were further submitted to an extended testing in various ovarian cancer cell lines (Table 2), since related derivatives showed good effects against A2780 ovarian cancer cells, especially Cisplatin-resistant subclones (A2780cis cells). 27,32 The cytotoxicity tests included besides the A2780/A2780cis cell lines also those enriched with cells with CSC characteristics (A2780V-CSC and IGROV1-CSC cells). 45 CSCs are characterized by clonogenicity, asymmetric division, and high tumorigenicity and thus are related to drug resistance and tumor relapse in diverse cancers. Hence,   46 Auranofin served as a reference and showed a comparable activity in ovarian A2780/A2780cis cancer cells as already published. 19,47 Compounds 5 and 6 were active with IC 50 values of 6−9 μM in A2780, A2780V-CSC, IGROV1, and IGROV1-CSC cells and 3−6 μM in A2780cis cells.
Complex 7 showed significantly higher effects due to the transformation in the medium, with the highest cytotoxicity in A2780cis (IC 50  The data listed in Table 2 show that A2780cis cells were high-sensitive to 8 and 9−11, with IC 50 = 0.02−0.10 μM. Cisplatin was 100-to 300-fold less active (IC 50 = 9.80 μM). Therefore, it seems to be possible to circumvent Cisplatinresistance with these compounds.
Especially, the activity in the low nanomolar range against the Cisplatin-resistant subclones is of interest. The resistance of A2780cis cells is triggered by an elevated ability to repair Cisplatin-damaged DNA. 48 In contrast to Cisplatin, which forms intrastrand cross-links at the DNA, the (NHC)Au(I)-X complexes 5−7 can only be mono-functionally bound to nucleobases. This could bypass the DNA repair system.
More difficult is the interpretation of the high cytotoxicity of 8 and its prodrugs 9−11. Complex 8 is inert against substitution reactions, and binding to bionucleophiles seems to be difficult. Nevertheless, Casini et al. 49 identified the Gquadruplex DNA as binding partner of [(NHC) 2 Au(I)] + complexes. This might be true as mode of action of 8−11.
Further targets are recently discussed by Augello et al. 11 for the 4-OCH 3 derivatives of 6 and 8. Interestingly, although higher antiproliferative, the [(NHC) 2 Au(I)] + complex did not inhibit the thioredoxin reductase, the well-accepted target of (NHC)gold(I) complexes. 16 Interference into other cellular pathways resulting in an increase of the intracellular ROS (reactive oxygen species) level, which in turn cause DNA damage, could also play an essential role.
These results clearly demonstrate that the complexes 7−11 are suitable for the treatment of cells with acquired and intrinsic resistance to common antitumor drugs. Especially, the high effects against CSC-enriched cell lines are noteworthy. Exceptionally, 7 was the most active complex against IGROV1-CSC cells.
Finally, it was evaluated in a preliminary experiment, whether the (NHC)Au(I)-GSH complex 12 possesses cytotoxic effects. It was incubated with A2780 cells and reduced the cell growth with IC 50 = 15.9 μM, which is about 2times higher than that of 5 and 6. Therefore, it is very likely that the GSH adduct is not part of the detoxification system of (NHC)Au(I)-X complexes. Detailed information will be published in a forthcoming paper.
Compounds 5 and 6, reduced the metabolic activity only at a concentration of 10 μM to 64 and 20%, respectively, while the same effect was achieved with 7 at 5-to 10-fold lower concentrations (metabolic activity 1.5 μM: 77%). Much higher was the influence of the cationic complexes 8−11. A significant reduction of the metabolic activity was already detected at the lowest concentration used (metabolic activity at 0.4 μM: 20− 30%). The IC 50 value represents the concentration causing a 50% decrease in metabolic activity after 72 h of incubation and is calculated as the mean ± SD of two or three independent experiments. An interaction with non-thiol containing amino acids was not evident. The role of GSH on the cytotoxicity of (NHC)Au(I)-X complexes is not fully elucidated. (NHC)Au-(I)-GSH is partially formed in the medium. Interestingly, this reaction did not lead to an inactive species. (NHC)Au(I)-GSH caused antiproliferative effects in A2780 cells, only 2-fold less than 5.
Regarding the antiproliferative effects of 5−11, it must be mentioned that only 5 and 8 were stable against nucleophiles of the cell culture medium and the biological activity can be ascribed to these species.
The nearly identical activity of 5 and 6 in all cell lines (IC 50 > 5 μM) resulted from the fast transformation of 6 to 5 in the cell culture medium, while partial ligand scrambling of 7 (→8) strongly increased the effects. The formed complex 8 was the most active compound in this study.
In the complete RPMI 1640 medium, supplemented with FCS, protein binding must be taken into account. Quantification of 7 and its degradation products in RPMI 1640/FCS indicated that about 50% of the available complex 7 is located in the protein bound fraction.
The complexes 9−11 showed another reaction profile, without ligand scrambling, but with halide exchange reactions.
Complexes 8 and 9−11 possessed high cytotoxicity and reduced the viability of, e.g., A2780cis cells in the low nanomolar range. The circumvention of Cisplatin-resistance in ovarian carcinoma cells is therefore feasible with these complexes. Remarkably, the complexes also showed a promising potential to eradicate therapy-resistant CSCs.
Unfortunately, selectivity for tumor cells is not given for 5− 11. The growth of SV80 fibroblasts was reduced to the same extent as tumor cells. Therefore, we will use in the following structure−activity relationship study the 1,3-dialkyl-4,5-diaryl-4,5-dihydro-1H-imidazol-2-ylidene as a new NHC carrier ligand to design effective (NHC)gold(I) complexes. The results will be presented in forthcoming papers.

■ EXPERIMENTAL SECTION
Chemical reagents and solvents were purchased from commercial suppliers (Sigma-Aldrich, BLDpharm, Fluka, Alfa Aesar, and Abcr) and were used without further purification. Analytical thin-layer chromatography was performed using Polygram SIL G/UV254 (Macherey-Nagel) plastic-backed plates (0.25 mm layer thickness) with fluorescent indicator and Merck TLC Silica gel 60 F254 aluminum-backed plates. The spots were visualized by UV light (254 nm). Column chromatography was done using silica gel 60 (0.040− 0.063 mm). NMR spectra were from a Bruker Avance 4 Neo spectrometer ( 1 H: 400 MHz, 13 C: 101 MHz). The center of the solvent signal and the TMS signal were used as internal standards. Deuterated solvents purchased at Eurisotop were used as solvents. HPLC experiments were performed using a Shimadzu prominence HPLC with an autosampler SIL-20A HT, column oven CTO-10AS VP, degassers DGU-20A, detector SPD-M20A, and pumps LC-20 AD with a KNAUER Eurospher 100-5 C18, 250 × 4 mm column. The software used for data processing was LabSolutions. Mass spectra were from an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) using direct infusion and electrospray ionization (ESI). MS data analysis was carried out with Xcalibur. The purity of all tested compounds was >95% as determined by HPLC analysis.
Synthesis and Characterization. Synthesis of the NHC-Ligand. 4,5-Diphenyl-1H-imidazole (2). Benzil (1) (2000 mg) was dissolved in 30 mL of conc. acetic acid together with 15 equiv of ammonium acetate (11.000 mg) and 1.1 equiv of paraformaldehyde (326 mg). The reaction mixture was then refluxed at 118°C. After 5 h, the solution was neutralized dropwise with saturated aqueous sodium carbonate and extracted three times with ethyl acetate. Removal of the solvent and precipitation with toluene afforded compound 2 (1953 mg, yield 93%) as a yellow solid. 1

1,3-Diethyl-4,5-diphenyl-1H-imidazolium Iodide (3).
To a stirred solution of 2 (1000 mg) and 1.1 equiv of sodium hydride (120 mg) in 30 mL of anhydrous ACN, 10 equiv of iodoethane (7081 mg, 3.65 mL) was applied and the reaction was stirred under an argon atmosphere at 82°C for 24 h in the dark. The solvent was removed under reduced pressure, and the residue was taken up in DCM and water. The aqueous phase was separated and washed three times with 20 mL of DCM, and the collected organic phases were dried over sodium sulfate and evaporated in vacuo to yield compound 3 (1802 mg, yield 98%) as a brownish solid. 1  1,3-Diethyl-4,5-diphenyl-1H-imidazolium Hexafluorophosphate (4). To exchange the iodide counterion for hexafluorophosphate, 1000 mg of 3 was dissolved in 5 mL of MeOH and 5 equiv of potassium hexafluorophosphate (2276 mg) in 5 mL of water was added dropwise. The desired product (4) precipitated, and the reaction mixture was stirred at rt for another 24 h. The precipitate was sucked off and washed three times with 10 mL of water. After drying in high vacuum, pure compound 4 (989 mg, yield 95%) was obtained as a yellow solid. 1  Chlorido [1,3-diethyl-4,5-diphenyl-1H-imidazol-2-ylidene]gold(I) (5). Complex 5 was synthesized according to the general method with lithium chloride (63 mg) as a chloride source. After chromatography and recrystallization with n-pentane, the pure complex 5 (90 mg, yield 60%) was collected as an off-white solid. 1  Iodido [1,3-diethyl-4,5-diphenyl-1H-imidazol-2-ylidene]gold(I) (7). 5 (30 mg) dissolved in 5 mL of anhydrous acetone was stirred together with 10 equiv of sodium iodide (88 mg) for 5 min at rt. The solvent was removed under reduced pressure, and the residue was dissolved in DCM and subsequently filtered through a pad of Celite to separate the remaining salts (NaI/NaCl) from 7. The filtrate was evaporated to dryness, and the residue was recrystallized from npentane to yield 7 (31 mg, yield 88%) as an off-white solid. 1 (8). 6 (59 mg) together with 1 equiv of 4 (45 mg) and 2 equiv of potassium carbonate (29 mg) was dissolved in 5 mL of anhydrous acetone under an argon atmosphere. The reaction mixture was stirred for 72 h under protection from light. Subsequently, the solvent was removed under reduced pressure and the crude product was purified by column chromatography (DCM/ethyl acetate = 1/1). Recrystallization from n-pentane yielded the pure complex 8 (77 mg, yield 87%) as an off-white solid. 1 (9). 8 (30 mg) was dissolved in 6 mL of DCM, and 10 equiv of dichloroiodobenzene (99 mg) was added. The reaction mixture was stirred at rt for 24 h. The solvent was removed under reduced pressure, and the residue was washed three times with 10 mL of diethyl ether and dried to yield the pure complex 9 (32 mg, yield 98%) as a yellow solid. 1 (10). To 30 mg of 8, 1.1 equiv of bromine (6 mg, 2 μL) was added under constant stirring to 6 mL of DCM at −30°C. Cooling was maintained for 30 min. Subsequently, the reaction mixture was stirred for another 2 h without cooling. The workup was carried out in the same way as described for 9 and yielded pure 10 (33 mg, yield 94%) as an orange solid. 1 (11). Complex 11 was synthesized according to the procedure described for 10 with the difference of using iodine (10 mg) instead of bromine. Red-orange solid, 37 mg (yield 94%). 1 (50/50, (v/v)) containing the double-concentrated amount of respective amino acid (20 mM), GSH (20 mM), sodium ascorbate (20 mM), or NADPH (20 mM). As a chloride source, an aqueous solution of KCl 0.8 g/L and NaCl 12 g/L was used. In each case, a final complex concentration of 0.5 mM was achieved. The solutions were incubated for 24 h at rt. For the experiments with RPMI 1640, the aqueous phase was replaced by a cell culture medium.
Samples (20 μL each) were taken at appropriate time points (t = 0 (1.5 min), 0.5, 1, 1.5, 2, 8, 12, and 24 h) and analyzed with a Shimadzu prominence HPLC system. The mobile phase consisted of ACN (HPLC-grade) and water (HPLC-grade) with 0.1% TFA. To achieve a separation of the compounds, gradient elution from (70/30 (v/v) to 90/10 (v/v)) of ACN/water (0.1% trifluoroacetic acid (TFA)) was used with a flow rate of 1 mL/min at an oven temperature of 35°C. All solvents have been degassed before use. The injection volume was 20 μL, and the detection wavelength was set at 235 nm, to detect the gold complexes and the amino acids in the chromatogram. Each experiment was displayed with the program Origin Pro 2018 (Origin LabCorporation, Northampton, MA, USA).
ESI-MS Measurements of 5 or 11 with GSH. 5 and 11, respectively, dissolved in MeOH were combined with an aqueous solution of GSH containing 0.2% formic acid, to get a 50/50 (v/v) solution with a complex concentration of 100 μM and a complex/ GSH proportion of 1/1 (5) and 1/10 (11).
The mixtures were measured on an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) using direct infusion and the HESI source in positive mode after 2 min and 24 h and analyzed with Xcalibur (Thermo Fisher Scientific, Waltham, MA, USA).
Determination of the FCS Influence on Stability of 7 in RPMI 1640. To 11 mL of RPMI 1640 including 10% FCS, 11 μL of a freshly prepared 3 mM DMF solution of 7 was added. The solution was then incubated for 4 h at 37°C in the dark. Proteins were precipitated with 33 mL of ACN. The mixture was cooled to −30°C and stored for 2 h at −20°C. Subsequently, the supernatant was separated by centrifugation (3000 rpm), collected, and dried by lyophilization. The lyophilizate was extracted three times with 5 mL of DCM and finally dried under reduced pressure. The residue was taken up in 1 mL of ACN, and 30 μL was analyzed by the HPLC method described above. Peak area was used for quantification. The calibrations of 5, 7, and 8 were obtained by injecting 30 μL of the respective solution of pure substances in ACN at a concentration of 1−30 μM (R 2 > 0.99).
Crystallography. A Bruker D8 Quest Kappa diffractometer equipped with a Photon 100 detector was used to collect the single-crystal intensity data. Monochromatized MoKα radiation was generated by an Incoatec microfocus X-ray tube (50 kV/1 mA power settings) in combination with a multilayer optic. The supplementary crystallographic data were deposited as CCDC 2180314-2180320 (5−11). Copies of the data can be obtained, free of charge, at the Cambridge Crystallographic Data Centre.
Biological Methods. Cell Lines. The ovarian carcinoma cell lines A2780 and A2780cis were kindly provided by the Department of Gynecology, Medical University Innsbruck. To maintain resistance, A2780cis cells were incubated every second week with Cisplatin (1 μM). The resistance of the A2780cis cells is caused by an increased ability to repair DNA damage mediated by cytogenetic abnormalities. 48 The fibroblast cell line SV-80, the lung cancer cell lines A549 and A549-R, and the ovarian cancer cell lines IGROV1, IGROV1-CSC, and A2780V-CSC were kindly provided by the Department of Internal Medicine V, Medical University Innsbruck. A549-R cells are resistant against Paclitaxel (5 nM). The resistance was generated by cultivating the A549 cells with gradually increasing concentrations of Paclitaxel. IGROV1-CSC and A2780V-CSC (originally termed IGROV1 SP and A2780V SP) represent therapy resistant cell lines with an enriched proportion of cancer cells with stem cell characteristics. 45 The CML cell line K562-R cells was originally described by Hui et al. as a Doxorubicin-resistant subclone of the K562 cell line (originally termed as KD225). 50 K562-R cells are also resistant toward Imatinib, as we described in previous publications. 51−54 The breast cancer cell lines MCF-7 and MCF-7TamR were purchased from DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. To maintain Tamoxifen resistance, the MCF-7TamR cells were treated with Tamoxifen (1 μM) every two weeks.
Analysis of Cell Growth Inhibition. Exponentially growing cells were seeded at a density of 1500 cells/well (A2780, A2780cis, A2780V-CSC, A549, and A549-R), 1750 cells/well (MCF-7 and MCF-7TamR), 2500 cells/well (IGROV1 and IGROV1-CSC), 3000 cells/well (SV-80), and 20,000 cells/well (K562 and K562-R), respectively, into clear flat-bottom 96-well plates in triplicates. After 24 h of incubation for adherent cell lines and 1 h of incubation for suspension cell lines (K562 and K562-R) at 37°C in a humidified atmosphere (5% CO 2 /95% air), the compounds were added to reach the indicated concentrations, respectively. The indicated final concentrations of the compounds in the well are 20, 10, 5, 2.5, 1.25, and 0.625 μM for substances 5 and 6 and the references Cisplatin and Auranofin, as well as 1.5, 0.75, 0.375, 0.1875, 0.09375, and 0.046875 μM for substances 7−11. All stock solutions were prepared in DMF at a concentration of 10 mM and were then diluted with RPMI 1640, supplemented with L-glutamine (2 mM) and FCS (10%), to the respective concentrations. After another 72 h of incubation, the cellular metabolic activity was measured employing a modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (EZ4U kit, Biomedica, Vienna, Austria) following the manufacturer's protocol. The optical density of the particular medium was subtracted to exclude the unspecific staining caused by FCS containing medium. The values were calculated with Excel 2019 (Microsoft, Redmond, WA, USA) using nonlinear regression and decal logarithm of the inhibitor versus variable slope equation, while the top constraint was set to 100%.